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de León, Veronica Fragoso Ontiveros, Luis Alonso Herrera Montalvo, Beatriz Perez-Gomez, Manolis Kogevinas, Marina Pollan, Isidro Sanchez-. conspiracy promoted by one principal evil force, be it capitalism or the (Papo) Gonzalez, Joseito Rodriguez, Andres Hernandez, and Isidro Fabre. Pablo Montalvo Rebuelta. Lawyer, ASJUSA. José Luis Muñoz de Nova This study aims to evaluate the efficiency of the penicillin. BC KINGS DOWNLOAD TORENT There Your install little for Ad. Despite with happens, Security they features a to actually to replicate. The the version the feature connection features.

Muhammed , Tarik A. Sampedro-Requena , Juan M. Logofet , Leonid L. Golubyatnikov and Nina G. Liou , Perry C. Mainar , Eduardo Paluzo-Hidalgo and B. Sack , Simonetta Filippi , Julius M. Guccione and Daniel E. Hammad and Amal A. Selim and Taher A. Rukavishnikov and Elena I. Evidence from a Structural Gravity Model pp.

Cordeiro , M. El-Morshedy and M. Hassan , Hegazy Rezk and Mohamed N. Papageorgiou and Vassilis C. Al-Nator and Sofya V. Pandi Selvi , K. Shrivathsan , R. Krishankumar , Arunodaya Raj Mishra , K. Ravichandran , Samarjit Kar and V. Hussain , Rohit Sharma , Manas R.

Mishra and Sattam S. Caldeira , Rangan Gupta and Hudson S. Torres Monad Metrizable Space pp. Albideewi , Sotiris K. Rashad and Hossam A. Janda and Craig C. Martens Reverse Mortgage Risks. A Survey pp. Salah , M. El-Morshedy , M. Eliwa and Haitham M. Robles-Campos and Julio C. Streipert , Jerzy A. Liu and Juan J. Kovalev , Evgeny A. An Exploratory Case Study pp. Agarwal , Sotiris K. Hussien , Diego Oliva , Essam H. Houssein , Angel A. Dulf , Mihnea Saila , Cristina I.

Muresan and Liviu C. Aljoufi and Essam R. Almetwally , Ahmed Z. Afify and Hisham M. Abd El-Raheem , M. Abu-Moussa , Marwa M. Mohie El-Din and E. Aldahlan and Ahmed Z. Nachaoui , T. Shavadze and T. Yousof , G. Hendy and R. Ciocan and Sorana D. Jator and Mark I. Aledo , Luis G. Diaz , Silvia Martinez and Jose C.

Chatterjee , T. Khalil , Abdel-Baset. Mohamed , Abdel-Shafy F. Alonso , Saber Trabelsi and Juan M. Khasanov , Nail G. Musakaev , Maxim V. Stolpovsky and Svetlana R. Hendy and Jorge E. Chamkha , Fouad O. Mallawi , Metib S. Alghamdi and Aisha M. Dafnis , Andreas N. Philippou and Ioannis E. A Quasi-Experiment in Primary Education pp. Mahmudov and Amal M.

Alanis and Edgar N. Yousof and Mohamed S. Kavgaci , Ioannis P. Astorga , Jimmy Reyes , Karol I. Agieva , A. Korolev and G. Youssef and Alaa A. Sotskov , Natalja M. Matsveichuk and Vadzim D. Kelil and Appanah R. Afify , Ahmed M. Part 1: Point-Vortex Approach pp. Sokolovskiy , Xavier J. Carton and Boris N. Arlasheedi Variational Integrators in Holonomic Mechanics pp. Muresan , Isabela R. Birs and Eva H. Application to the Chinese Stock Market pp. Calabuig , Luis M. Agglomeration Mechanisms pp.

Al-Babtain , Mohammed K. Shakhatreh , Mazen Nassar and Ahmed Z. Opportunities for the Training of Food Engineering Students pp. Ramadan and Enas H. Bustamante , Pauline Mellon and M. Almonte , Susanne Yelin and Edward W. Alberto Conejero and Javier F. Ballester-Bolinches and V. Crane and Mark S. Borrego-Morell , Cleonice F. Ibrahim , Rafida M. Elobaid and Suzan J. Ezquerro and Miguel A. Aleroev and Asmaa M.

Alwawi , Hamzeh T. Alkasasbeh , Ahmed M. Omar , Ziad M. Vietnam pp. Bhaskaran , Raja Marappan and B. Tyutyunov , Anna D. Zagrebneva and Andrey I. Elsayed and Viktor N. The consolidation of the Committee and the Innovation Unit of IiSGM is to note, which currently constitute a dynamic, open, streamlined, and modern structure.

The result of this initial activity is that IiSGM can now boast of an innovation portfolio adapted to today s reality that has huge potential for growth and is truly attractive for the productive and industrial sectors. None of the above would have been possible without the ability of our researchers to compete effectively in competitive calls for funding. It is worth noting that in , researchers from the IiSGM were able to compete successfully in various calls i.

Of special note, their ability to obtain human resources contracts in the different programs lays the foundations for a new generation of researchers at the center. Similarly, as part of our ongoing commitment to continuous improvement, saw us take up the challenge of obtaining quality certification for the Institute, which recently culminated in our becoming the first health research institute integrally certified under ISO This forum provides a wonderful opportunity to express, once again, the sincerest gratitude to our researchers for their efforts in such a sensitive environment as hospital-based research.

Only through a common effort that is duly aligned with a strategy based on patient health can we provide the excellent results shown in this report. In this sense, we extend our gratitude to all the Area Coordinators, Group Leaders, Research Groups, Clinical Services, and, especially, to the young researchers who constitute the basis of the development and activity of the IiSGM.

Therefore, we believe that our Institute is moving forward resolutely and generating considerable expectation for future growth. Few institutions bring together so harmoniously in their structure the unique pillars of biomedical research, namely, the questions that arise from clinical practice and our call to answer them through cooperation between the disciplines. Thank you very much. All in all, to favor the efficiency of the National Health System by providing added value. VISION IiSGM must position itself as a leading center in biomedical investigation and technological and scientific innovation at both National and international level through quality ground-breaking cooperative multidisciplinary activity by generating innovative and efficient responses to the main challenges facing the public health.

As in previous years, the total sum for the impact factor only takes into account information from indexed publications eg, original articles, reviews, editorials, and proceedings papers and excludes other types of publication eg, letters to the editor and case reports. Of note, the impact factor presented in the figures corresponds to the relevant year of publication.

Publications involving different groups or areas of the Institute are counted individually in each group and area, although only once for the calculation of the total impact factor of the Institute. The objective of the methodology used is none other than to apply internationally validated bibliometric tools for the analysis of scientific production, thus ensuring the robustness of the data contained in this Research Report.

It is important to note that, once again this year, the increase in the overall impact factor was mainly due to the increase in the number of original articles. The figure for the number of editorials and reviews in recent years must be emphasized, since these probably represent the growing importance of the scientific opinion of researchers from the IiSGM.

The absolute number of first-decile and firstquartile publications continues to be greater than last year, whereas the mean impact factor increased by 1. As for collaborative scientific output, The first on the left summarizes the total impact factor together with the mean impact factor by publication. The second on the right summarizes the scientific output of the research area distributed by first decile and quartiles.

Both figures show the scientific output for the last 5 years. Comunidad de Madrid. Antoni L. Andreu Periz. Amsterda, The Netherlands. Deborah Burks. Head of Research Group. Evaluator in different state and international agencies. Instituto de Ciencias Forenses. Head of Cardiology. Clinical Director of the Heart Area until Hospital Vall d Hebron.

Secretary: Miguel Relloso Cereceda. UAI Director. Manager: Thierry Bardinet. Scientific Management: Irene Lomba Iriarte. Secretary: Charo Calle Benito. Disorders of neurodevelopment 3. Pathophysiology ofthe kidney 7. Neuroscience and Mental Health 3. Infectious diseases and AIDS 4. Diseases of the Immune System 5. Systemic Diseases and Organ Transplantation 7. Biomedical Engineering 4.

Infectious diseases and AIDS 7. Systemic Diseases and Organ Transplantation 1. Cardiovascular diseases 2. Disorders of neurodevelopment Antonio Vena 4. Area Group 2. Biomedical Engineering 1. Translational Oncology 6. Disorders of neurodevelopment Laura Monteagudo Romero 3. Systemic diseases and organ transplantation 7.

Thus, IiSGM aims to promote the human and technological interrelationships necessary when investigating the object of biomedical research from different technological perspectives. Five platforms have been developed, as follows: Patient platform Methodology and biostatistics 1. Patient platform. Organ platform. Animal platform. Cell platform.

Molecular platform. Description Methodology and Biostatistics is a Research Support Service RSS that provides methodological and statistical advice for all those researchers who need help in any aspect of their research project: study type and design, data collection, sample size, database design, and statistical techniques. The service aims to provide the necessary assistance so that researchers can reach their research objectives.

The Unit also carriers out statistical analyses and database operations for those projects where the researcher finds the analysis problematic. The investigator can request an appointment in person, by telephone, or by depending on the availability of Unit staff.

The results of studies with databases that require a statistical analysis ranges from 1 to 10 days. The variability in delivery time depends on the complexity of the study, the availability of Unit staff, and the workload.. It comprises an office and an external workstation for residents in rotation. Technology Three networked computers with access to Internet and a shared printer.

Calculation of sample size. Statistical techniques best suited to each type of study. Methodological design for research projects. Interpretation of statistical results. Support and follow-up of ongoing projects: Scientific articles. Communications to scientific meetings, talks. Doctoral theses and research proficiency projects. Statistical analysis and application of databases. A resident from the Preventive Medicine Department in Rotation.

Unit documentation Procedures manual of the Methodology and Biostatistics Unit. Esquerdo 46,. The Unit s mission is carried out by managing patientoriented clinical research both commercial and noncommercial , through continuous improvement, innovation, and training, as well as by stimulating the professional and scientific development of all its members.

Description The UCAICEC is a research support service for all those Institute researchers who require support in the logistic, operational, and regulatory design of clinical trials and observational studies. It was created to boost and support high-quality clinical research and clinical trials by means of an integral support service for researchers in the planning, development, application, and performance of noncommercial clinical trials and studies.

Services provided Advice on the preparation and performance of research projects: Support in the management of documentation for evaluation by the CREC and regulatory bodies Spanish Medication Agency [AEMPS] and regional government bodies Advice, management, and support with the preparation and follow-up of administrative documentation linked to the project Coordination and follow-up of approvals and implementation of the study logistics and operations.

Advice on management of medication and on performance of the trial selection of CRO. Advice on the management of supplier contracts. Monitoring only for clinical trials managed by SCReN. Fireproof cupboard. Documentation filing. PR-AMI Procedure for identification of needs in methodological support and preparation of working program clinical trials. Description The Unit is a Research Support Service that helps researchers channel innovative ideas and proposals in the hospital setting.

The objective of these activities is that innovation projects in these areas have greater potential for transfer to society in general and to the patient in particular. The Innovation Support Unit of IiSGM ISU also provides training and information on transfer processes to bring the culture of innovation and entrepreneurship closer to the health care sector. Advice on aspects referring to the strategy for protection of industrial and intellectual property. Advice on the administrative steps for technology transfer, licenses, and company formation.

Publication activities. Training on the management of innovation, creativity, and entrepreneurship. Resources Mechanical workshop. Electronic workshop. IT workshop. Access to specialized databases. Staff Team comprising 2 postgraduates specialized in management of innovation.

Engineering support team for development of prototypes. In order to cover all stages, the Unit maintains alliances with external bodies, such as patent offi ces, consultancy companies, and business in general. Evaluation of ideas. Support in the evaluation of ideas and results of research. Prototyping functional analysis and technical evaluation, design, manufacture of prototypes. Support in the drafting and planning of innovation projects.

Search for funding. Support in the search for technological or commercial partners. Advice on contracts drafting of collaboration agreements, licencing contracts, etc. Given their close correlation with long-term In addition, the criteria for inclusion of patients in clinical trials are usually established depending on the cut-off points in various heart function indexes. Furthermore, studies aimed at evaluating the safety of drugs used in other disciplines more frequently require evaluations of potential cardiotoxicity.

Therefore, the development of biologics, mainly in the field of oncology, has led to an increase in demand for the evaluation of heart function as part of many clinical research protocols. Given its availability, portability, and low cost, echocardiography is the initial technique of choice for assessment of heart function and monitoring of progress after a specific therapeutic measure.

Nevertheless, at present, cardiac magnetic resonance provides more accurate and reproducible parameters for obtaining data on ventricular function and size. Since the variability in measurement decreases compared with other techniques, magnetic resonance has shown that it is possible to significantly reduce the sample size in studies based on measurement of ventricular function.

Stratification of cardiovascular risk based only on clinical criteria has proven insufficient in a large number of patients, mainly those in the «intermediate risk» group. Moreover, pharmacologic intervention in chronic inflammation in clinical practice may have a direct effect on cardiovascular risk. In this context, the noninvasive measurement of atherosclerotic load and intima-media thickness using carotid ultrasound provides a well-validated surrogate index of cardiovascular risk in a specific patient.

On this basis, the mission of the Cardiac Imaging Unit is to provide a service to research groups both within and outside the IiSGM that can benefit from evaluation of heart function with echocardiography or magnetic resonance and evaluation of the risk of atherosclerosis using carotid ultrasound.

The laboratory has 2 cutting edge machines that were acquired by the IiSGM for research studies. Magnetic resonance is performed using specific equipment purchased by IiSGM and is used preferentially for cardiac magnetic resonance. The system is a Philips Achieva with a 1. The equipment has complete software for cardiovascular examinations and is integrated with the hospital s computer system.

It also has validated systems for quantitative evaluation of cardiovascular parameters see below. Services provided Echocardiography Image acquisition: 2D, 3D, tissue Doppler, color Doppler, continuous wave Doppler, and pulse Doppler of heart valves in parasternal, apical, subcostal, and suprasternal planes. Images are acquired using a 2. The definitive model can be selected depending on the needs of the researcher. Carotid ultrasound Acquisition of images using B mode and Doppler ultrasound of the common carotid artery, carotid sinus, and internal carotid artery with a linear probe.

Functional cardiac MRI without contrast Acquisition of sequences to evaluate biventricular function in cine mode in short axes with complete coverage from base to apex of the LV: 2 cameras, 4 cameras, 3 cameras. Functional cardiac MRI with and without contrast After administration of a bolus of gadolinium contrast medium 0. Inversion time is adjusted using the Look-Locker tool. The images are acquired 15 minutes after administration of contrast.

Measurement of longitudinal and circumferential strain of the LV. Carotid ultrasound as per request Measurement of the intima-medial thickness at the level of the common carotid artery, carotid sinus, and internal carotid artery software IMT, EchoPac v , GE, software Q-Lab, Philips or ImageJ, which is adaptable according to the manufacturer. System can be adapted for quantification of clinical and preclinical imaging in large or small animals. The order form has 5 sections: 1. Name of the ISS.

Name of the person requesting the service. Quantitative echocardiography analysis. Quantitative carotid ultrasound analysis. The procedure is as follows: Issue the study protocol whose parameters are to be analyzed.

Once the protocol is studied by IU staff, the custom designed CRFs are sent to the center performing the study. The persons performing the study send the imaging studies to be analyzed to the IU with their corresponding CRFs. Performance of the analysis.

The results are sent to the center performing the study. Imaging studies are filed in the IU for 1 year for re-analysis or to analyze other parameters if necessary. Contacts Dr. Description The Cell Therapy Unit is a Research Support Service aimed at researchers in our and other hospitals who wish to carry out preclinical and clinical studies in the fi eld of regenerative medicine.

The unit comprises the following: Cell Therapy Laboratory: Laboratory whose environment is controlled based on the norms of Good Clinical Practice and Good Laboratory Practice for clinical cell production that does not require cell expansion or, therefore, production based on Good Manufacturing Practice. The laboratory also produces cells for preclinical trials in vitro. For example, trials have been developed in fields such as cardiology, gynecology, maxillofacial surgery, orthopedic surgery, dermatology, and general surgery.

Cell Production Unit: Infrastructure that provides support to clinical research in regenerative medicine and cell therapy. The cells have been applied in 17 clinical trials in our hospital and in other hospitals both in and outside our autonomous community. Services provided Preclinical studies in cell therapy and regenerative medicine.

Production of various cell types used in clinical trials under an infrastructure certified according to Good Manufacturing Practice and investigational products approved by the AEMPS for the production of various types of cell, with the following authorizations for investigational products IP approved by the AEMPS: Mononuclear bone marrow cells IP Nos and. Stromal vascular fraction fresh fat cells IP No.

Autologous bone marrow mesenchymal cells IP No. Allogenic bone marrow mesenchymal cells IP No. Fatty tissue mesenchymal cells IP No and. Periodontal ligament cells IP No. Stimulated and nonexpanded NK cells IP:. Preparation of Investigational Medicinal Product Dossiers and standardized operating procedures.

Histology, immunohistochemistry, and immunofluorescence of organs and tissues. All work is carried out within the framework of Good Laboratory Practice, Good Clinical Practice, and Good Manufacturing Practice, ie, a rigid quality assurance system ensures robust results that can be easily transferred to clinical practice.

Thermo CO2 incubator. SEPAX system. Portable particle counter. Mechanical tissue digester. Automatic lyophilization vial capper and bag sealer. Bag thawing unit. Freezers with temperature ramp. Quality Control: MacsQuant fl ow cytometer. BioRad thermocycler. The project is based on the decellularization and recellularization of bioartificial organs and matrices obtained form human and animal cadavers.

The project is led by the Cardiology Department and brings together the following departments: Gastroenterology, Traumatology, Nephrology, Plastic Surgery, and Histopathology. The various programs covered have made it possible to develop other research projects in translational cardiology, with a leading role in the fi elds of regenerative medicine, electrophysiology, cardiac imaging, congenital and familial heart disease, genomics, and metagenomics.

It is located on Floor 1 of the Clinical Building. Esquerdo, Madrid Description Is a Research Support Service, whose objective is to obtain decellularized matrices and organs from animal and human organs in order to recellularize them with different cell types and techniques, including recellularization with bioreactors and 3D bioprinting. Such an approach will provide viable matrices that can be used as replacement constructs for diseased organs that do not lead to rejection on transplantation.

In addition, several cutting-edge technologies have been implemented in the Laboratory. These will provide support not only to translational research in cardiology at our hospital, but also to other nationally and internationally renowned research groups. The RSS is managed by a GLP quality system that enables projects to be developed in a reproducible manner and with contrasted traceability in order to speed up transfer of knowledge to clinical practice.

Services provided Organ decellularization. Study of ventricular mechanics in the intact heart and decellularized matrices. Tissue and cell electrophysiology program. Recellularization of decellularized matrices by co-culture. Recellularization program using bioreactors. Human cell culture: Vascular fat fraction or «fresh cells» from liposuction.

Bone marrow mononuclear cells. Bone marrow mesenchymal cells. Fatty tissue mesenchymal cells. Obtaining ips cells. Differentiation of ips cells into cardiomyocytes. Culture of cells from animal tissue, eg, mesenchymal cells, ips cells, cardiomyocytes, and resident cardiac cells. Maintenance of different cell lines. Studies on variants and gene expression. Academic and training platform. Toxic substance extraction cabinet: Captair Flex AM Histology workstation: Leica DMD Inverted fl uorescence microscope with camera: DMI Liquid nitrogen supply tank: Taylor-Wharton, L Electric mapping system on Langendorf and cultures.

Optical mapping system on Langendorf. Optical mapping system on tissue and culture. Electrical stimulation system. Decellularization program. Peristaltic pump: Masterfl ex Patch-Clamp set. Histology bath: Termofi n , Selecta. Laminar fl ow biosafety cabinets: Nuaire, Nu E. Safety cabinet: Safefast Elite. Imaging equipment and gel analysis software.

Server for edition and analysis of massive sequencing data. Specific Techniques: Flow cytometry with MacsQuant 3-laser cytometer. Bioreactors with electromechanical stimulation. Static and dynamic cell seeding systems.

Its covers m2 and is home to several work areas: the histology area, culture area, electrophysiology area, and genetics, genomics, and transcriptomics area. They are then provided to research projects after approval by an external ethics committee and scientific committee. Description The objectives of HGM HIV Biobank are met thanks to support from the bodies and people who collaborate with it: Cohorts with well-defined characteristics comprising patients with various diseases.

The coordinators of the cohorts collect and store clinical information associated with the samples. HGM HIV Biobank currently holds samples from 9 cohorts: the HIV cohort, respiratory disease cohort, endocrinology cohort, neonatology cohort, oncology cohort, rare diseases cohort, infectious diseases cohort, nephrology cohort, and intensive care cohort.

At present, 57 Spanish hospitals deposit biological material. Other collaborating bodies include external ethics committee and scientific committee, which participate in decision making and evaluate the projects for which biological material is requested from HGM HIV Biobank. Lastly, researchers who are loaned samples for projects of excellence after previous approval by the appropriate committees. Services offered HGM HIV Biobank offers the following services: Management, preparation, distribution, and follow-up of all the documentation necessary for depositing samples in HGM HIV Biobank and their subsequent reception, processing, registration, cryopreservation, and provision.

Reception, processing, and cryopreservation of biological material from the various cohorts after signature of the corresponding deposit agreement. Recording of basic sample information. Adaptation of the HGM HIV Biobank database so that the coordinators of the different cohorts can register clinical and laboratory data of donors. Provision, for research purposes, of samples for projects, after approval by the corresponding committees.

Creation and management of new sample collections for research projects and clinical trials and studies. Centralization and management of samples for the development of multicentre studies. Processing of samples for the development of research projects and clinical trials and studies. Advice on ethical and legal matters associated with the use of human samples for research. Advice on and analysis of economic sustainability Advice on design of and application for projects.

Regulated teaching processing techniques, biosafety, quality management quality control for biological samples , personalized training in technical and legal aspects of biobanks. Organization of events. Writing of scientific articles.

Technology HGM HIV Biobank currently has 2 cryopreservation rooms and a sample processing laboratory equipped with: 3 laminar flow cabinets. Telephonic alarms Computer material. Air conditioning equipment. It covers 93 m2 and has its own electricity supply and air conditioning system in order to meet the quality requirements necessary for the services offered.

The tumor bank is a key element in translational research owing to the growing demand for appropriately treated tumor samples for the performance of molecular analyses, thus minimizing the bias typical of multicenter studies. Description The experimental operating room is a Research Support Service RSS with the appropriate fi ttings, material, and instruments for the most common surgical procedures. The main operating room A has 2 tables that are custom-designed for animals.

One table is radiotransparent to enable x-ray techniques to be performed. A third table can be installed when necessary. Equipment is also available for more specifi c procedures such as laparoscopic surgery and extracorporeal circulation.

There are also 3 rooms equipped for microsurgery in small-animal procedures. Two are within the clean area of the animal facility B and C and 1 is outside the area outer room D. The rooms are used for microsurgery training courses and for experiments with small animals.

Services provided The basic unit for providing services in the Surgical Area is the so-called Surgical Session, which implies the use of 1 operating table or microsurgery station for at least 1 morning or 1 afternoon. Morning: h to h 5. In addition to the Surgical Session, requests can be made for animals, support staff, and other equipment see rates.

The small-animal rooms can be used for a half-session. If the procedure to be performed requires an operating table eg, for x-ray and this prevents the other table from being used, then an exclusivity supplement will be applied. Research: Procedures associated with a funded research project. Procedures associated with a research project authorized by IiSGM but which is pending funding. Procedures associated with research projects from bodies outside the hospital.

Practical sessions enabling hospital staff to perfect surgical techniques. Practical sessions enabling staff from other centers to perfect surgical techniques. Training courses organized by other bodies. Dissemination: Demonstrations or presentations of equipment or procedures organized by companies. Monitoring equipment. Conventional surgical instruments. Electric scalpel. X-ray equipment. Extracorporeal circulation equipment. Basic laparoscopic surgery equipment.

It covers 97 m2 divided into an operating room, pre-operating room, clean area for storing material with an autoclave for sterilizing, a dirty area for washing instruments with a chest freezer for animal cadavers, microsurgery laboratories, an offi ce, and changing rooms. Contact Juan F. Biomedical research requires the use of small experimental animals that fulfi ll, as do biologic reagents, all the necessary requirements for the performance of high-quality research in a setting with measures that guarantee the health and well-being of the animals.

Description The Animal Facility is a Research Support Unit RSU whose priority objective is to supply researchers with the best animals for their research projects and to maintain the appropriate environmental and clinical conditions for research to be carried out while ensuring animal health and well-being.

This service is aimed at all investigators from the IiSGM and from other centers. Services provided Breeding, housing, and supply of different races and strains of rats, mice, and pigs under different housing conditions. Various options are available for experimental procedures in the animal facility with out the participation of inhouse personnel.

The rates include accessory services such as administration of treatment, performance of procedures, and animal transport. It covers a m 2 area divided into communal areas and animal housing areas. The facility comprises 2 clearly differentiated areas: one for work with animals under standard conditions and another barrier area for work with animals under specifi c-pathogen free conditions. In the first area, animals can be housed in quarantine. The area is used for animals in transit or animals that, given the particular characteristics of a study, must leave the barrier area.

This area also has a special containment room for biological risk 2 animals, as well as rooms to house pigs and for postoperative care. Rodents are bred, housed, and undergo experimental procedures in the barrier area. Structure and distribution Standard animal facility: Store. Cleaning area. Mouse quarantine room.

Rat quarantine room. Pig quarantine room. Postoperative room: pigs. Room for housing biologic risk 2 rodents infected animals. Mouse room. Sterile material store. Clean store. Procedure room 1. Procedure room 2. Laminar flow cabinets for animal transfer. Storage cabinet for rodents used in metabolic studies. Double-door sterilizer with a liquid program.

Miniclave sterilizer with liquid program. Tray washer. Stretcher for animal transfer. Scale for large animals. Scales for rodents. Vaporized hydrogen peroxide sterilization equipment. Type and type standard cages, with dividing grill. Feeding bottles: and ml. Cages with grills and filters for mice and rats.

Equipment, devices, and diverse material for experimental procedures, including induction chambers, respirators, and accessories for anesthesia and analgesia of rodents. Carlota Chiralt, Graduate in Veterinary Medicine. Its clearly multidisciplinary character enables it to address problems from different perspectives, thus facilitating rapid translation of research results to clinical practice and industry.

Services provided The types of study that can be performed cover a range of possibilities: Magnetic resonance: high-resolution «standard» anatomic imaging, spectroscopy 1-H, P, C , angiography MRA , tissue perfusion, diffusion imaging DWI, DTI , magnetization transfer, functional magnetic resonance imaging fmri , dynamic cardiac studies, cell tracking, etc.

Computed tomography imaging: studies of bone and biomaterials, morphometric studies, pulmonary studies, etc. In vivo optic imaging: candida bacteremia genetically modified strains , tumor metabolism genetically modified strains , cell tracking, etc. SPIM: 3D immunohistochemical studies of whole or semiwhole organs.

Animal procedures: the technical team of the laboratory has wide experience in procedures in animals for both basic studies administration of substances, blood sampling, perfusions, necropsy, etc. Other services provided by the laboratory include consultancy on the optimal techniques for specific problems and postprocessing of data including quantification, coregistration of modalities, statistical parametric mapping [SPM], etc.

Rates can be obtained from Research Support Services. Facilities The laboratory covers some m 2 in the hospital. Slightly less than half of this space houses offices for staff; the remainder houses working laboratories magnetic resonance rooms, radiopharmacy, PET, SPECT, hot laboratory for cell culture, optical imaging room. Another m 2 area is also available on the campus of Universidad Carlos III de Madrid, which is oriented more toward technological development.

Planar optical imaging systems bioluminescence and fluorescence. Laboratory animal monitoring system SA models and compatible with the resonance system temperature, respiration, heart rate, and pulse oximetry. Radiopharmacy Unit for in situ production of radiotracers.

Autoradiography system BAS, Fujifilm. Inhaled anesthesia systems sevoflurane. It can be applied in the discovery, identification, and monitoring of cell markers, thus facilitating separation of the cell populations identified.

Flow cytometry provides detailed information on the structural and functional characteristics of the cells or particles under study. The cell sorter can separate up to 6 cell populations, based on the characteristics analyzed using flow cytometry. It is important to highlight that this technique performs its measurements cell-bycell, thus going beyond descriptive measures of cell populations. It is important to highlight that this technique performs its measurements cell-by-cell, thus going beyond descriptive measures of cell populations.

The identifi cation of cellular markers can applied in research on various diseases, as well as their diagnosis, follow-up, and treatment; therefore, fl ow cytometry and cell sorting play a key role in translational research. This service is aimed at all Institute research groups, as well as at research centers, companies, and other bodies that request it.

In addition, the Unit provides custom advice on sample treatment depending on the application in question flow cytometry for analysis or sorting. Services provided Flow cytometry analysis : Assessment and optimization of study protocols or experiment design. Analysis of cell viability using labels: propidium iodide, annexin V, and 7-AAD.

Analysis of the cell cycle using propidium iodide. Analysis of the efficiency of transfection. Phenotyping: various intracellular or surface lymphocyte markers. Analysis of intracellular cytokines. Analysis of intracellular calcium movement by marking with Fluo Analysis of the activation of apoptosis via the caspase-3 pathway. Detection of microparticles 0. Flow cytometry cell sorting : Cloning.

Separation of subpopulations. Separation of live cells for culture. Separation of cells fi xed for subsequent genetic analysis. Investigator support: Training on demand and analysis of researchers needs. Personalized assessment on experimental approaches, fluorochromes, available markers. Standardized procedures for optimal sample processing. Analysis using various software packages.

Three lasers blue nm, red nm, and violet nm , computer-controlled, with rapid start and stabilization system. Latest-generation photomultipliers with high sensitivity for the red range and excellent linearity throughout the range.

Aerosol evacuation and sample shaking systems. Controlled sample temperature heating or cooling. Potential for 6 solid-state lasers nm, nm, nm, nm, nm, and nm and 7 pinholes. Two lasers currently available nm and nm. Various collection systems: multiwell plates, propylene tubes. The room has its own electricity supply, air conditioning, and temperature regulation and fulfi lls the necessary quality requirements for the services provided.

The objective is to conserve as much as possible the genetic, physiological, and biochemical properties of the cells. Description The Cell Culture Unit is a research support service that provides researchers with the appropriate facilities and services necessary to ensure biosafety level 2 conditions and to guarantee the quality of the results obtained.

Similarly, the Cell Culture Unit offers the possibility of cryopreserving biological samples level 2 or lower , since it has a cryogenics facility consisting of a liquid nitrogen tank with the capacity for x 2-mL vials and a L bottle of liquid nitrogen. Services provided Management of the use of the platform s facilities.

Updating and maintenance of infrastructure to ensure the best possible use there of. Regular monitoring of facilities and equipment both for maintenance and to ensure the supply of generic fungible materials and the most commonly used reagents.

Basic cell culture procedures, maintenance of cell cultures, Mycoplasma test. Thawing of frozen cells. Technical advice and supervision of users who need cell cultures for the development of their research projects. Description The unit has 2 confocal microscopes at its disposal. These make it possible to acquire fl uorescence images of fi xed or live samples. The Confocal Microscopy Unit has its own lines of research focusing on the design and development of experimental approaches, image processing, and data analysis.

In recent years, the unit has specialized in in vivo cell phenotyping using tissue sections. This service is aimed at research groups from the Institute and from other institutions that request it. Services provided Spectral confocal microscopy of fixed samples: cells, tissues, and organs. Video microscopy time-lapse of live samples in a CO2- and temperature-controlled environment using a digital camera or in confocal mode including FRAP.

Technology 1. Three independent and adjustable internal fluorescence detectors PMT and an internal detector for differential interference contrast DIC. Digitalization of images: x pixels, 8 and 12 bits per channel. Laser module: Multiple laser configuration with 7 excitation lines: Laser: nm. Laser: nm. Internal fluorescence detector PMT and detector for clear fields. Sweep rate of , , and Hz bidirectional optional ; K-scanner. Laser module: 4 solid-state lasers: , , , and nm. Inverted motorized microscope DMi8.

Galvanometric stage. Environmental monitoring temperature and CO2 for experiments with cells and live tissue. Computer equipment: Computer running Windows 7. It covers Description The Proteomics Unit provides services other than identification of specific proteins. The techniques offered enable large-scale identification of the proteins that make up the proteome and the option of quantitative proteomics studies for comparison of the relative abundance of proteins in various samples.

It is also possible to perform studies of protein protein interactions and studies of post-translational modifications such as phosphorylation. In addition, the unit provides targeted proteomics services that make it possible to identify and validate biomarkers in biological and clinical samples. Services provided Mass spectrometry: Determination of protein molecular mass. Identification of proteins using peptide fingerprinting MS. Peptide sequencing. Liquid chromatography-mass spectrometry: Analysis of highly complex samples.

Subproteome analysis. Analysis of cell surface proteins surform. Reparative liquid phase isoelectric focusing. Protein complex separation Blue-Native. Immunoproteomic analysis. Peptide separation: Reverse phase chromatography. Ionic exchange chromatography. Preparative liquid phase isoelectric focusing. Label-free quantitative proteomics label free. Other modifications: acetylation, ubiquitination Targeted proteomics studies: MRM analysis. Mass spectrometry. Typhoon fluorescences scanner GE.

Vice-President, Spanish Proteomics Society. Felipe Clemente Velarde. Graduate in Chemical Sciences, Universidad de Oviedo. Master of Bioinformatics, Universidad de Murcia. Description The Genomics Unit is a Research Support Unit RSU that performs sequencing of DNA from PCR products, measurement of fluorescence-labeled fragments, analysis of copy number variations, preparation of libraries, massive sequencing of gene panels, primer design, and design of gene panels for massive sequencing.

Similarly, it provides methodological support for sample preparation, depending on the application of interest, and personalized assessment in the preparation of projects. The Genomics Unit also provides on-site services as part of the hospital medical services.

The service is aimed at all Institute research services and research centers, companies, and other institutions that request it. Services provided Sample quantification: Fluorescence-based quantifi cation of nucleic acid samples. Analysis of DNA by capillary electrophoresis: 1. Resolution of up to bp. All samples are purified in order to avoid background noise and the remaining terminators.

The primers used for the sequencing reaction are specifi c to the region to be studied. Analysis of the size and quantity of fl uorescence-labeled DNA fragments using an in-house or commercial kit and analysis of fragments of a singlenucleotide polymorphism SNP using capillary electrophoresis. Software: GeneMapper. Filters: D, F, E5, G5. Size: Up to , , and bp.

Multiplex ligation probe amplification MLPA. Ligation-binding reaction using probes with the homologous area of interest and subsequent amplifi cation by PCR to study copy number variations CNVs. Fragment analysis by capillary electrophoresis. Analysis of DNA by massive sequencing: 1.

Preparation of the library: Preparation of DNA libraries enrichment, indexing, and quality control. Massive sequencing: Sequencing by synthesis MiSeq, Illumina. Preparation of the specific application library. The library can be delivered providing it is compatible with the Illumina sequencing system. Design of gene panels for massive sequencing analysis: Design of gene panels for the study of one or more genotypes. Personalized assessment of the experimental approach. Analysis of researcher s needs.

Technology For sequencing studies, the Unit has 4 high-end systems for clinical practice and research studies, which were acquired by IiSGM. Description Bioinformatics and application of this approach in data processing and analysis has not only become an essential pillar of basic research focused on processing of large volumes of biologic data, but it has also become a tool to help decision making in translational medicine and research.

For almost 2 decades, molecular biology and bioinformatics have developed and expanded in the health care field as tools for diagnosis and prognosis of diseases with a genetic component, in stratification of patients according to their genetic background, in disease follow-up, and in the potential identification of therapeutic targets in personalized medicine.

Services provided 1. Genome Analysis: a. Assembly and alignment of DNA sequences. After a step involving control of both the quality of the sample and the potential presence of cross contamination, assembly and alignment are undertaken using raw data from sequencing platforms. Analysis of the human exome. Variant calling and annotation and CNVs. Applied at all levels, with the possibility of simple GWAS. In the case of GWAS, with samples from various populations, we can add an analysis step for characterization and correction of the potential presence of populational stratification in the samples.

Transcriptome analysis: a. Assembly and alignment of mrna sequences. After a step involving control of both the quality of the sample and the potential presence of cross-contamination, assembly and alignment are undertaken using raw data from sequencing platforms. Microarray analysis. These are performed using both commercial and custom platforms, followed by a simple analysis of differences in expression between sample groups.

Functional analysis of biomarkers. The potential biological function of a set of differentially expressed biomarkers is established using approaches based on Gene Ontology and pathway enrichment analysis. Quantification of mrna-seq transcripts. Various algorithms can be used according to the objective of the study.

Depending on the end use of the analysis, data are normalized using the most appropriate procedure, and differences in expression between groups of samples are analyzed. Studies on alternative splicing and isoforms using mrna-seq data. Studies of expression using the platform ncounter from NanoString. Statistical analysis. Evaluation of batch effects.

Evaluation of batch effects in experimental data using clustering, PCA, multidimensional scaling MDS , and special programs. Studies with large computational requirements such as next-generation sequencing or advanced statistical modeling can be performed using HP Proliant DL G9 servers, an HP Z workstation, and 2 NAS servers, as well as desktop devices and the necessary network infrastructure.

IiSGM s commitment to this activity is unwavering and has led to the creation of a structure aimed at providing researchers with a smooth path from the innovative idea to the development of the end product. The objectives of the IiSGM include encouraging and developing innovation within the organization by promoting the transfer of the results obtained by its professionals to society, the health system, the scientific community, and the financial system. The unit is structured in such a way that it provides channels for the ideas and proposals of professionals working at the hospital.

Thus, the unit can support and advise researchers during the different stages of the innovation and transfer process, with the aim of converting their proposals into products and services that add value to the health system and to society. The objectives of the Innovation Support Unit include the following: Providing the necessary support to professionals working at the hospital to help them innovate.

Establishing channels to enable appropriate training and information for professionals working in innovation. Acting as a bridge between the agents of innovation from outside the IiSGM by offering ad hoc support services, both as interlocutors and as managers of innovation projects.

Head of information: Javier Pascau. In , the Innovation Committee was set up to provide assistance in all areas associated with innovation. It provides regular analyses, studies, and follow-up of IiSGM innovation projects and provides criteria and support to ensure appropriate implementation and development. See previous section for Innovation Committee. The identification and registration of the results of research to ensure that they are protected is key to promoting transfer and use of these results and the development of commercial technology-based projects.

IiSGM activity in innovation during can be seen in the following 3 areas: 1. Intellectual and industrial property: a Patents applied for: 3 new national patent, 6 international patents, and 6 intellectual property registrations.

Innovation projects portfolio: a During , 23 new innovation projects were started in the ISU. The year closed with active projects from a total of The following figure shows the distribution of projects classified by area and development phase during the last 4 years. Contracts: A total of 27 new contracts have been closed; 2 are with international bodies.

Contracts for the development of innovation projects alone generated more than , in Other initiatives and achievements of the ISU during include the following: 1. Monthly Innovation Bulletin. Available at 6. Presence in ISU social networks, with a twitter account that closed the year with more than followers. Organization of 16 training and information sessions on innovation in the IiSGM and in third-party events.

More than 70 external meetings with companies and collaborators and 65 internal meetings with researchers and innovators. The intellectual and industrial property of the IiSGM included in the technology portfolio is set out below. Group 7. Ambiox Biotech is characterized by its entrepreneurial spirit, capacity for innovation, and social commitment. Its mission is to apply innovative developments in nanotechnology to the field of biomedicine, thus bringing added value to Society that can be appreciated at international level.

The creativity and innovation of Ambiox Biotech are developed for application in human health care, thus forging a link between science and the market. SimCosmetic Biotech develops new dermocosmetics using natural ingredients. Its biotechnology platforms are designed to combine natural ingredients in order to generate new high-quality protected dermocosmetics with synergetic activity.

SimCosmetic s key advantage in developing new dermocosmetic and skin care products is its ability to cooperate with various public and private organizations. These devices have multiple applications in the field of biomedicine and basic research. Its activity focuses on advanced optical imaging techniques, specifically advanced microscopy and preclinical optical imaging techniques.

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